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notch3ecd antibody  (Novus Biologicals)


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    Novus Biologicals notch3ecd antibody
    Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti <t>NOTCH3ECD</t> antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant
    Notch3ecd Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch3ecd+antibody/pm26912635-114-5-7?v=Novus+Biologicals
    Average 90 stars, based on 4 article reviews
    notch3ecd antibody - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Therapeutic NOTCH3 cysteine correction in CADASIL using exon skipping: in vitro proof of concept."

    Article Title: Therapeutic NOTCH3 cysteine correction in CADASIL using exon skipping: in vitro proof of concept.

    Journal: Brain : a journal of neurology

    doi: 10.1093/brain/aww011

    Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti NOTCH3ECD antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant
    Figure Legend Snippet: Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti NOTCH3ECD antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant

    Techniques Used: Ligand Binding Assay, Transfection, Incubation, Expressing, Staining, Cell Culture, Luciferase, Activity Assay, Plasmid Preparation, Co-Culture Assay



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    Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti <t>NOTCH3ECD</t> antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant
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    Image Search Results


    Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti NOTCH3ECD antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant

    Journal: Brain : a journal of neurology

    Article Title: Therapeutic NOTCH3 cysteine correction in CADASIL using exon skipping: in vitro proof of concept.

    doi: 10.1093/brain/aww011

    Figure Lengend Snippet: Figure 4 Ligand binding and ligand-induced signalling of NOTCH3 skip proteins. (A) Fibroblasts were transfected with NOTCH3 cDNA and incubated with a jagged 1-Fc peptide to allow for ligand binding. The jagged 1-Fc peptide was preclustered to an anti-Fc antibody (red fluorescence). The NOTCH3 expressing cells are visualized using an anti NOTCH3ECD antibody (green fluores- cence). Cells transfected with wild-type NOTCH3 cDNA show an increased jagged 1 staining where the NOTCH3 protein is ex- pressed, whereas this staining is absent in the cells transfected with NOTCH3 lacking the ligand binding domain (del EGFr 10–11). The cells expressing the NOTCH3 skip proteins all show an increased jagged 1 staining similar to wild-type NOTCH3, indicating normal ligand binding. Images are shown from one representative experi- ment at a 40 magnification. (B) NIH3T3 cells transiently trans- fected with NOTCH3 cDNA, were co-cultured with jagged 1 expressing cells to induce NOTCH3 signalling, and assayed for luciferase activity using a hexamerized CBF1 reporter plasmid. Cells transfected with wild-type NOTCH3 cDNA show a significant in- crease in luciferase activity upon co-culture with 3T3 cells ex- pressing human jagged 1, compared to co-culture with mock transfected 3T3 cells not expressing human jagged 1. The cells transfected with the respective skip NOTCH3 cDNA also showed a significant increase in luciferase activity. There was no significant

    Article Snippet: Cells were stained the anti NOTCH3ECD antibody (Novus Biologicals, dilution 1:1000) and with an anti-actin, -smooth muscle antibody (Sigma-Aldrich, dilution 1:500) to confirm the smooth muscle cell phenotype (Supplementary Fig. 2).

    Techniques: Ligand Binding Assay, Transfection, Incubation, Expressing, Staining, Cell Culture, Luciferase, Activity Assay, Plasmid Preparation, Co-Culture Assay